Delta2D Expression Profile

Profile

Quantities

The table shows normalized volumes (%Vol) of the visible expression profile for spot ID '13850' on gel image 'control_01'.
Ratios and p-values (using t-Tests) are computed relative to group 'control'.

control 1 min 10 min

control_01 control_02 1min_01 1min_02 10min_01 10min_02

ID 13850 13850 13850 13850 13850 13850

Label EF-G (no label) (no label) (no label) (no label) (no label)

X 708 645 632 617 630 650

Y 135 122 137 115 133 142

%V 2.8054 2.3024 2.5876 2.1050 0.8575 0.7283

Ratio - 0.919 0.310

p-value - 0.612 0.021

RSD 9.847 10.283 8.152

mean 2.554 2.346 0.793

median 2.554 2.346 0.793

maximum 2.805 2.588 0.858

minimum 2.302 2.105 0.728

	control	1 min	10 min

ID	13850	13850	13850	13850	13850	13850
Label	EF-G	(no label)	(no label)	(no label)	(no label)	(no label)
X	708	645	632	617	630	650
Y	135	122	137	115	133	142
%V	2.8054	2.3024	2.5876	2.1050	0.8575	0.7283
Ratio	-	0.919	0.310
p-value	-	0.612	0.021
RSD	9.847	10.283	8.152
mean	2.554	2.346	0.793
median	2.554	2.346	0.793
maximum	2.805	2.588	0.858
minimum	2.302	2.105	0.728

Labels

Scout	Attribute	control	Fused Images
		control_01	Fused Image
Label name(s)		EF-G	EF-G
Pi/MW Estimation	Pi	N.A.	N.A.
	MW	N.A.	N.A.
GenoList	Gene Size (bp)	0	0
	Protein Size (aa)	0	0
	Function
Uniprot	Accession	P80868
	Amino Acids	692
	Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).
	Gene name	fusA
	Gene Ontology	C:cytoplasm [GO:0005737] C:intracellular [GO:0005622] F:GTP binding [GO:0005525] F:GTPase activity [GO:0003924] F:translation elongation factor activity [GO:0003746]
	Isoelectric Point	4.5136
	Keywords	Complete proteome Cytoplasm Direct protein sequencing Elongation factor GTP-binding Nucleotide-binding Phosphoprotein Protein biosynthesis Reference proteome
	Molecular Weight	76616.6
	NCBI Taxonomy	224308
	Organism	Bacillus subtilis (strain 168)
	Original label name	EF-G
	Protein name	Elongation factor G
	Query text	EF-G
	References	6 references
	Sequence	MAREFSLEKTRNIGIMAHIDAGKTTTTERILFYTGRIHKIGETHEGASQMDWMEQEQERG ITITSAATTAQWKGYRVNIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVW RQATTYGVPRIVFVNKMDKIGADFLYSVGTLRDRLQANAHAIQLPIGAEDNFEGIIDLVE NVAYFYEDDLGTRSDAKEIPEEYKEQAEELRNSLIEAVCELDEELMDKYLEGEEITIDEL KAGIRKGTLNVEFYPVLVGSAFKNKGVQLVLDAVLDYLPAPTDVAAIKGTRPDTNEEIER HSSDEEPFSALAFKVMTDPYVGKLTFFRVYSGTLDSGSYVKNSTKGKRERVGRILQMHAN SREEISTVYAGDIAAAVGLKDTTTGDTLCDEKDLVILESMEFPEPVIDVAIEPKSKADQD KMGIALAKLAEEDPTFRTQTNPETGQTIISGMGELHLDIIVDRMKREFKVEANVGAPQVA YRETFRTGAKVEGKFVRQSGGRGQFGHVWIEFEPNEEGAGFEFENAIVGGVVPREYIPAV QAGLEDALENGVLAGFPLIDIKAKLFDGSYHDVDSNEMAFKVAASMALKNAVSKCNPVLL EPIMKVEVVIPEEYMGDIMGDITSRRGRVEGMEARGNAQVVRAMVPLAEMFGYATALRSN TQGRGTFTMHMDHYEEVPKSVAEEIIKKNKGE
	Title	EFG_BACSU
	Uniprot entry	P80868

	control		1 min		10 min
	control_01	control_02	1min_01	1min_02	10min_01	10min_02

ID	13850	13850	13850	13850	13850	13850
Label	EF-G	(no label)	(no label)	(no label)	(no label)	(no label)
X	708	645	632	617	630	650
Y	135	122	137	115	133	142
%V	2.8054	2.3024	2.5876	2.1050	0.8575	0.7283
Ratio	-		0.919		0.310
p-value	-		0.612		0.021
RSD	9.847		10.283		8.152
mean	2.554		2.346		0.793
median	2.554		2.346		0.793
maximum	2.805		2.588		0.858
minimum	2.302		2.105		0.728

Profile

Quantities

Labels

Position on Gel Image 'control_01'