Can I use Delta2D to analyze 1D gels?

It depends. Delta2D was developed and optimized for 2D gels. One-dimensional gels have different spot forms so you might have to use the spot editing more extensively. If the order of the lanes does not change between your gels, you can use Delta2D's warping to align different gels and produce expression profiles the way you would for 2D gels. In general, you'll probably be better served by a software package that is specifically developed for 1D gels, such as LabImage 1D.